Cord levels of zinc and copper in relation to maternal serum levels in different gestational ages

Abstract

Background

A few published data on maternal and cord levels of zinc and copper with conflicting results were reported. We aimed to measure zinc and copper levels in the maternal blood and cord blood of newborns and correlate their levels with the gestational age and anthropometric measurements. This cross-sectional study included 75 full-term and preterm neonates and their mothers. These neonates were divided into 2 groups according to their gestational age. Serum levels of copper and zinc were estimated for the mothers of the studied neonates as well as their cord samples. This was done using atomic absorption spectrophotometry.

Results

The mean cord serum zinc in full term was 0.88 ± 0.18 μg/ml whereas in preterms was 0.73 ± 0.13 μg/ml. The mean cord copper in full term was 1.37 ± 0.26 μg/ml, whereas in preterms was 0.75 ± 0.28 μg/ml. Comparison between cord zinc and copper levels and maternal levels were statistically significant (p < 0.001). A significant positive correlation was found between zinc levels in neonates and their mothers (r 0.644; p < 0.000) and a similar positive correlation was found between copper levels in neonates and their mothers (r 0.625; p 0.000). A highly significant positive correlation was found between cord zinc and copper and anthropometric measurements.

Conclusion

The present work draws our attention to the significant correlation between trace elements in pregnant mothers and fetal development. Also, prematurity adversely influences zinc and copper levels in neonates.

Background

Trace elements are vital nutrients that cannot be synthesized in the human body [1].

They are important components for transcription factors and function as catalytic cofactors for enzymes regulating cell differentiation and maturation [2].

Zinc and copper are important trace elements that are necessary for optimum human growth and development [3]. Zinc is protective against free radicals and is involved intracellularly in cell division and nucleic acid synthesis. Copper is also an essential cofactor in oxidative and reductase enzymes [4].

Deficiencies of trace minerals have been reported, yet review of literature has not revealed clear recommendations regarding their supplementation [1]. The maternal mineral status during gestation is essential for growth and development of the fetus and subsequent neonatal heath [5].

Since preterm infants are born prior to the time of rapid intrauterine fetal growth, they are vulnerable to deficiencies of essential nutrients for brain development, including zinc and copper [6]. Therefore, these elements need to be studied to supply health personnel with fundamental data necessary for appropriate management.

There are a few published researches on maternal and cord levels of zinc and copper with inconsistent results. The purpose of this study was to measure zinc and copper levels in the maternal blood and cord blood of newborns and to correlate their levels with the gestational age and anthropometric measurements.

Methods

Patients and settings

This cross-sectional study was conducted over a period of 18 months’ duration. The study included 75 full-term and preterm neonates of either gender and their mothers. These neonates were divided into 2 groups according to their gestational age. Group 1 consisted of 35 full-term neonates > 37 weeks of gestation and their mothers, while group 2 consisted of 40 preterm neonates < 37 weeks of gestation and their mothers. Babies with congenital anomalies such as hydrocephalus, sacral agenesis, or myopathies were excluded from the study as these may affect the anthropometric measurements. Also, babies of mothers with medical problems such as maternal diabetes and hypertension were excluded. An informed consent was taken from the mothers. The study was approved by the Ethics Committee of Pediatric Department, Faculty of Medicine.

Data gathering

All cases were subjected to assessment by full maternal and neonatal history taking. A detailed maternal history included the age of the mother, parity, previous pregnancies, last menstrual period, complications of pregnancy such as toxemia of pregnancy and antepartum hemorrhage, antenatal vitamin supplementation, and mode of delivery.

Full thorough examination of the neonates was done, and all included neonates had normal chest, cardiac, abdominal, and neurological examination, with good neonatal reflexes and Apgar score.

All the neonates’ measurements were taken on birth day including birth weight (kg), birth length (cm), and head circumference (cm) using the tape, measuring the greatest circumference of head passing by the glabella.

Clinical assessment of gestational age was done according to New Ballard score.

Serum levels of copper and zinc were estimated for the mothers of the 75 studied neonates as well as cord samples from them.

Biochemical parameters

Blood samples were collected using sterile dry disposable syringes for measurement of copper and zinc at the time of birth. Samples were kept at room temperature and left to clot for 30–60 min. Then, tubes were centrifuged at 1500 rpm for 15 min and separated serum was directly transferred to be kept frozen at – 20 °C till time of assay. Strict measures to avoid contamination of the samples were taken.

Determination of serum copper and zinc levels was done by atomic absorption spectrophotometry using Varian SpectrAA 220 Flame Atomic Absorption Spectrometer (Varian, Australia). The spectral lines used for determination were 213.9 nm for Zn and 324.7 nm for Cu. The standard sources of the investigated elements were hollow cathode discharge lamps made by Cathodeon, England. The recommended slit widths used for element determination were 1.0 and 0.5 nm for Zn and Cu, respectively. The fuel used for the flame was acetylene for the two elements under investigation with oxidizing flame stoichiometry. The oxidant (air) flow rate was 3.5 l/min and the fuel rate (C₂H₂) was 1.5 l/min.

Serum samples were diluted five times with double distilled water just before sample measurement. All solutions were prepared with ultrapure water with double distilled water commercially available. One thousand-milligram per liter Zn and Cu standard solutions were used (Tritisol, Merck) in order to prepare the working standard solutions. These were freshly prepared and calibration graphs were constructed for each element with suitable standard samples according to the recommendations of the manufacturer of the instrument.

To detect possible interference and matrix effects, the correlation of the absorption with the existing concentration was made by applying the normal calibration graph. No matrix interference for zinc and copper was noted, as it can be deduced from the similarity of slopes corresponding to the two methods used (linear calibration and addition calibration method). No chemical interferences were measured, while measuring the elements of interest in this work. An aliquot of 100-μl sample was aspirated to measure the absorption signal using a homemade Teflon micro-funnel as explained elsewhere.

Statistical analysis

After collection of the data, they were coded, entered, and processed on computer using Statistical Packaged for Social Science (SPSS version 16, 2007). The level P < 0.05 was considered the cut-off value for significance. Chi-Square test χ² was used to test the association variables for categorical data. Fisher exact test was performed in table containing value less than 5. Student’s t-test was used to assess the statistical significance of the difference between two population means in a study involving independent samples. The correlation coefficient method was used to relate different parameter. Linear regression analysis approach to modeling the relationship between a scalar dependent variable and one or more explanatory variables was used. Graphic presentation of the results was also done.

Results

A total of 150 subjects were included in this study, 40 preterm neonates and their mothers and 35 full-term neonates and their mothers. The study included 46 females (61.3 %) and 29 males (38.7 %).

The mean gestational age of the full terms was 39.1 ± 0.85 weeks, and their mean birth weight was 2.96 ± 0.19 kg while the preterms had a mean gestational age of 33.6 ± 2.5 weeks and a mean birth weight of 1.69 ± 0.43 kg. Thirty-two full-term neonates and 17 preterms were delivered by normal vaginal delivery while 3 full terms and 23 preterms were delivered by Caesarean section.

The mean cord serum zinc level in full-term neonates was 0.88 ± 0.18 μg/ml, whereas in preterms was 0.73 ± 0.13 μg/ml. Also, the mean serum zinc level in mothers of full term was 1.09 ± 0.15 μg/ml, whereas in the mothers of preterms was 0.96 ± 0.15 μg/ml.

The mean cord serum copper in full-term neonates was 1.37 ± 0.26 μg/ml, whereas in preterms was 0.75 ± 0.28 μg/ml. Also, the mean serum copper level in mothers of full terms was 1.69 ± 0.39 μg/ml, whereas in the mothers of preterms was 1.40 ± 0.33 μg/ml.

Comparison between cord serum zinc and copper levels and maternal levels proved to be statistically significant (p < 0.001) (Table 1).

Table 1 Comparison between serum zinc and copper levels in cord and maternal blood of preterm and full-term neonates

A significant positive correlation was found between zinc levels in neonates and their mothers (p < 0.05); an increasing level of zinc in mothers was associated with an increasing level of zinc in their neonates (r 0.644; p 0.000). A similar positive correlation was found between copper levels in neonates and their mothers (r 0.625; p 0.000) (Figs. 1 and 2).

Fig. 1

Correlation between zinc level in neonates and zinc level in mothers

Fig. 2

Correlation between copper level in neonates and copper level in mothers

A significant difference in both cord copper and zinc was detected in full-term neonates among appropriate (AGA) and small for gestational age (SGA). However, among preterm neonates, cord copper was significantly higher in appropriate for gestational age in comparison to small for gestational age with no difference in cord zinc between the 2 groups (Table 2).

Table 2 Comparison between cord copper and zinc levels in AGA and SGA preterms and full terms

A significant positive correlation was found between cord zinc and copper levels and gestational age. A highly significant positive correlation was found between cord zinc and copper levels and birth weight, birth length, and occipital frontal circumference (Table 3).

Table 3 Correlation of cord zinc and copper levels with the anthropometric measurements in the studied neonates

However, no significant relation was found between zinc levels and sex or mode of delivery. Cord copper levels had a significant relation to the mode of delivery (p < 0.05) with no relation to sex.

The maternal serum zinc and copper showed a positive correlation with gestational age and anthropometric measurements of neonates, so higher maternal copper and zinc were associated with larger anthropometric measurements (Table 4).

Table 4 Correlation of maternal serum zinc and copper levels with the anthropometric measurements in all the studied neonates

Among the mothers of the studied neonates, 45 (60%) were on multivitamin and mineral supplementations during pregnancy, while 30 (40%) were not. Babies of supplemented mothers had statistically significant higher anthropometric measurements than babies of non-supplemented mothers (p < 0.00). However, owing to the low socioeconomic status of the mothers and their inaccurate reporting of the type and duration of supplements, these results are questionable.

In our study, univariate and multivariate linear regression analysis were performed to evaluate the factors affecting weight of neonates. In the stepwise linear regression analysis, the insignificant independent variables were removed one by one (maternal serum zinc and copper level). Other than gestational age, the only variable, neonatal zinc and copper, was independently associated with birth weight. The regression coefficient was (zinc = 0.450, 95% confidence interval was 1420, and copper = 0.379, 95% confidence interval was 1.009) (P = 0.000).

Discussion

Trace elements are essential for all biological cell function. They play an important role during pregnancy and deficiencies during gestation may adversely affect both mother and fetus [7]. Essential elements in pregnant women and the fetus are affected by the major physiological changes occurring during pregnancy, in addition to the other significant factors such as maternal age, week of gestation, nutritional status, and living environment [8].

In the current study, the cord blood levels of copper and zinc in preterm neonates were significantly lower in comparison to full-term neonates. A highly significant positive correlation of serum copper and zinc levels with gestational age (r = 0.305 and p = 0.008) was observed.

The lower serum copper levels in preterms may be attributed to its increased need for fetal growth and development and its important role in erythropoiesis. This is also intensified by the immaturity of the liver to synthesize ceruloplasmin. Preterm and low-birth weight babies have low zinc levels due to deficient body stores, as 60% of fetal zinc is acquired during the last trimester of pregnancy [9].

Jafaar et al. reported increasing zinc levels with increasing gestational age [10]. Also, Boskabadi et al. in Japan reported lower cord blood zinc and copper in preterms relative to full-term neonates [11]. On the contrary, no significant difference was found between preterms and full terms in the study done by Sakha et al. in Iran [12]. On the contrary, Kojima et al. found lower zinc levels with increasing gestational age [6].

A significant relation was found between cord copper and zinc levels and the anthropometric measures. Full-term neonates showed higher levels of cord blood copper and zinc in AGA in comparison to SGA. However, in the preterm group, AGA showed significantly higher serum copper levels than SGA with no difference in serum zinc levels. So more mature babies had higher zinc and copper levels and the higher the level, the greater were the anthropometric measurements indicating more growth.

Amalia et al. reported that zinc and copper levels in normal neonates were significantly higher than in neonates with fetal growth restriction. Fetal growth restriction can occur due to placental insufficiency which causes defects in placental circulation and transport affecting nutrient transport [13]. The results of Zadrozna et al. research in Poland found a decrease in zinc levels by 37% and copper by 27% in neonates born with fetal growth restriction [14].

Ofakunrin et al. [15] and Abbas et al. [16] demonstrated a significant positive association between cord zinc concentration and birth weight, which suggests that zinc is an important element for fetal growth and development. Sultana et al. in agreement with our work showed a positive correlation between serum copper and mean birth weight of preterm and full-term neonates [17].

On the other hand, Kojima et al. [6] reported no difference in zinc levels in SGA than AGA preterm neonates similar to our results but they found higher copper levels in SGA rather than AGA. A study done in Bangladesh by Islam et al. [18] reported no significant difference in serum zinc level between preterm SGA and AGA babies. Also, Xiang and colleagues reported that serum zinc level in umbilical cord blood had no statistically significant effect on SGA and LGA [19].

Contrary to this work, evaluation of ten trace elements by Bermudez and colleagues showed that cord copper was the only one inversely and independently related to birth weight with highest concentrations found in the SGA group [20]. Marriott et al. reported no significant associations between height, weight, and head circumference with trace element levels except for a weak correlation between copper at term and head circumferences [21].

In the present study, comparing the maternal and neonatal levels revealed that the maternal serum copper and zinc levels were higher than the cord copper and zinc levels. A positive correlation was found between maternal and neonatal zinc and copper levels (r = 0.644 and p = 0.000; r = 0.625 and p = 0.000, respectively).

The higher maternal copper levels can be explained by the transfer of stored copper in maternal tissues (specially the liver) to the growing fetus. Also, the concentration of ceruloplasmin is elevated during gestation with an increased level in maternal blood than in cord blood [22]. Bermudez et al. also reported a positive correlation between cord copper and maternal serum levels [20].

However, Tsuzuki et al. [23] and Ofakunrin et al. [15] reported no significant difference between neonatal and maternal zinc and copper concentrations.

Other studies [5, 22, 24] found lower concentrations of copper and a higher level of zinc in cord blood than in maternal blood. The reduction in maternal zinc levels may be explained by numerous factors including elevated zinc uptake by the fetus and placenta, increased mobilization of plasma zinc to maternal erythrocytes, physiological dilution secondary to enhanced maternal blood volume, and reduced serum albumin for zinc binding during pregnancy. In addition, zinc transporters, as ZnT, present in the placenta, accelerate zinc uptake from the maternal blood to the fetus. Also, previous studies done by Sakha et al. [12] and Iqbal et al. [25] reported significantly lower maternal zinc levels in comparison to their neonates.

Lower zinc and copper in mothers of preterms was found in relation to zinc and copper of mothers of full terms. This is also confirmed by Sultana et al. [17] in Bangladesh who suggested that during pregnancy high levels of estrogen increase ceruloplasmin resulting in increased serum copper in full-term mothers.

Also Maamouri and colleagues stated the presence of a significant association between low birth weight and maternal zinc levels [4]. Many researchers around the world who found a positive correlation between maternal serum zinc and birth weight reported mothers with lower zinc levels to be 2.6 times more liable to deliver LBW babies than those with normal levels, and neonates with decreased zinc levels were 2.8 times more at risk of being LBW [10, 26, 27].

In the present study, there was no relation between the sex and level of serum zinc and copper. Zych and colleagues reported that neonatal gestational age, gender, and birth weight had no substantial impact on concentrations of trace elements in the cord blood except concentrations of iron which was statistically significantly correlated with gender of neonates [28].

In our study, univariate and multivariate linear regression analysis were performed to evaluate the factors affecting weight of neonates. Other than gestational age, only neonatal zinc and copper were independently associated with birth weight.

Conclusion

The present work draws our attention to the significant correlation between trace elements in pregnant mothers and fetal development through analysis of the anthropometric measurements of neonates. In addition, prematurity adversely influences zinc and copper levels in neonates. These results may help in establishing guidelines for supplementation of trace elements.

Limitation

This study had several limitations; the population size needs to be larger in order to observe better results in subgroups and we did not analyze the dietary intake and nutritional status of pregnant women which may affect maternal concentrations of trace elements.